RMC PT-XL Ultramicrotome can be used for the preparation of ultra-thin sections for electron microscopy. Automatic sectioning thickness range is from 50 nm up to 1 µm. This unit is equipped with a stereo microscope for a clear view of the sample and knife edge. Biological samples have to be chemically fixed, and embedded in a resin block. The ultra-thin sections, usually with lateral dimensions of ~ 1 mm x 1 mm, can be collected on a TEM grid for electron microscopy. It is also possible to collect the thin sections on a microscope glass slide for optical microscopy.
Glass Knife Maker
The GKM-2 glass knife maker uses commercial glass strips with normal length of 200 mm and 6 mm in thickness. Glass squares are first produced from glass strips by breaking the strip into 25 mm glass squares. The squares are scored nearly exactly diagonally, as close to 45° as possible, to create knives of maximum sharpness for the microtome sectioning.